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human il 18 levels  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd human il 18 levels
    Human Il 18 Levels, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 13 article reviews
    human il 18 levels - by Bioz Stars, 2026-08
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    cytokine levels  (Cusabio)
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    ANXA6 promotes the inflammatory phenotype of macrophages by exacerbating macrophage pyroptosis. (A) qPCR was performed to analyze CD86, IL-12, CD206, and CD163 mRNA levels (n=3). (B) Detection of inflammatory <t>cytokine</t> secretion by ELISA kits (n=5). (C) Fluorescence images of CD86 (n=3). (D) Quantitative values of the MFI of CD86 (n=3). (E) Representative flow cytometry results of CD86 (n=3). (F) Representative fluorescence images of CD206 (n=3). (G) Quantification of the MFI of CD206 (n=3). (H) Representative flow cytometry results of CD206 (n=3). (I) Examination of pyroptosis of each group of cells by TUNEL staining (n=3). (J) The number of TUNEL-positive cells was quantified (n=3). (K-L) NLRP3, ASC, caspase-1, and GSDMD-N protein levels were assessed by Western blotting (n=3), and the statistical value was analyzed. (M-N) qPCR analysis revealed mRNA expression of inflammatory <t>cytokines</t> <t>IL-1β</t> and IL-18 (n=3). (O-P) Protein levels of IL-1β and IL-18 were quantified by ELISA (n=3). (Q) The activity of caspase-1 (n=3). Student's t-test was used to assess differences between the two groups. * P < 0.05, ** P < 0.01.
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    ANXA6 promotes the inflammatory phenotype of macrophages by exacerbating macrophage pyroptosis. (A) qPCR was performed to analyze CD86, IL-12, CD206, and CD163 mRNA levels (n=3). (B) Detection of inflammatory <t>cytokine</t> secretion by ELISA kits (n=5). (C) Fluorescence images of CD86 (n=3). (D) Quantitative values of the MFI of CD86 (n=3). (E) Representative flow cytometry results of CD86 (n=3). (F) Representative fluorescence images of CD206 (n=3). (G) Quantification of the MFI of CD206 (n=3). (H) Representative flow cytometry results of CD206 (n=3). (I) Examination of pyroptosis of each group of cells by TUNEL staining (n=3). (J) The number of TUNEL-positive cells was quantified (n=3). (K-L) NLRP3, ASC, caspase-1, and GSDMD-N protein levels were assessed by Western blotting (n=3), and the statistical value was analyzed. (M-N) qPCR analysis revealed mRNA expression of inflammatory <t>cytokines</t> <t>IL-1β</t> and IL-18 (n=3). (O-P) Protein levels of IL-1β and IL-18 were quantified by ELISA (n=3). (Q) The activity of caspase-1 (n=3). Student's t-test was used to assess differences between the two groups. * P < 0.05, ** P < 0.01.
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    AS-IV inhibits pyroptosis in podocytes treated with high glucose. (a) Effect of YVAD (Z-YVAD-FMK) on cell survival in podocytes exposed to high glucose. (b) The effect of AS-IV on expression of IL-1 β and <t>IL-18</t> in podocyte supernatant. (c) TUNEL staining to detect DNA fragmentation in podocytes in vitro (200x magnification, n = 3). Scale bar: 100 μ m. (d) PI (propidium iodide)/Hoechst 33342 staining (200x magnification, n = 3). Scale bar: 50 μ m. (e) The effect of AS-IV on LDH (lactate dehydrogenase) release in podocyte supernatants. (f) Caspase-1/PI staining as assayed by flow cytometry. (g) GSDMD-N and caspase-1 immunofluorescence (630x magnification, n = 3). Scale bar: 75 μ m. (h) The effect of AS-IV on pyroptosis-related parameters in podocytes exposed to high glucose. Values are presented as mean ± SD ( n = 3, ∗ P < 0.05 and ∗∗ P < 0.01).
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    il 18 protein levels  (Boster Bio)
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    AS-IV inhibits pyroptosis in podocytes treated with high glucose. (a) Effect of YVAD (Z-YVAD-FMK) on cell survival in podocytes exposed to high glucose. (b) The effect of AS-IV on expression of IL-1 β and <t>IL-18</t> in podocyte supernatant. (c) TUNEL staining to detect DNA fragmentation in podocytes in vitro (200x magnification, n = 3). Scale bar: 100 μ m. (d) PI (propidium iodide)/Hoechst 33342 staining (200x magnification, n = 3). Scale bar: 50 μ m. (e) The effect of AS-IV on LDH (lactate dehydrogenase) release in podocyte supernatants. (f) Caspase-1/PI staining as assayed by flow cytometry. (g) GSDMD-N and caspase-1 immunofluorescence (630x magnification, n = 3). Scale bar: 75 μ m. (h) The effect of AS-IV on pyroptosis-related parameters in podocytes exposed to high glucose. Values are presented as mean ± SD ( n = 3, ∗ P < 0.05 and ∗∗ P < 0.01).
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    ANXA6 promotes the inflammatory phenotype of macrophages by exacerbating macrophage pyroptosis. (A) qPCR was performed to analyze CD86, IL-12, CD206, and CD163 mRNA levels (n=3). (B) Detection of inflammatory cytokine secretion by ELISA kits (n=5). (C) Fluorescence images of CD86 (n=3). (D) Quantitative values of the MFI of CD86 (n=3). (E) Representative flow cytometry results of CD86 (n=3). (F) Representative fluorescence images of CD206 (n=3). (G) Quantification of the MFI of CD206 (n=3). (H) Representative flow cytometry results of CD206 (n=3). (I) Examination of pyroptosis of each group of cells by TUNEL staining (n=3). (J) The number of TUNEL-positive cells was quantified (n=3). (K-L) NLRP3, ASC, caspase-1, and GSDMD-N protein levels were assessed by Western blotting (n=3), and the statistical value was analyzed. (M-N) qPCR analysis revealed mRNA expression of inflammatory cytokines IL-1β and IL-18 (n=3). (O-P) Protein levels of IL-1β and IL-18 were quantified by ELISA (n=3). (Q) The activity of caspase-1 (n=3). Student's t-test was used to assess differences between the two groups. * P < 0.05, ** P < 0.01.

    Journal: International Journal of Biological Sciences

    Article Title: ANXA6 Overexpression Causes Abnormal Decidual Macrophage-Trophoblast Crosstalk in Recurrent Spontaneous Abortion

    doi: 10.7150/ijbs.111791

    Figure Lengend Snippet: ANXA6 promotes the inflammatory phenotype of macrophages by exacerbating macrophage pyroptosis. (A) qPCR was performed to analyze CD86, IL-12, CD206, and CD163 mRNA levels (n=3). (B) Detection of inflammatory cytokine secretion by ELISA kits (n=5). (C) Fluorescence images of CD86 (n=3). (D) Quantitative values of the MFI of CD86 (n=3). (E) Representative flow cytometry results of CD86 (n=3). (F) Representative fluorescence images of CD206 (n=3). (G) Quantification of the MFI of CD206 (n=3). (H) Representative flow cytometry results of CD206 (n=3). (I) Examination of pyroptosis of each group of cells by TUNEL staining (n=3). (J) The number of TUNEL-positive cells was quantified (n=3). (K-L) NLRP3, ASC, caspase-1, and GSDMD-N protein levels were assessed by Western blotting (n=3), and the statistical value was analyzed. (M-N) qPCR analysis revealed mRNA expression of inflammatory cytokines IL-1β and IL-18 (n=3). (O-P) Protein levels of IL-1β and IL-18 were quantified by ELISA (n=3). (Q) The activity of caspase-1 (n=3). Student's t-test was used to assess differences between the two groups. * P < 0.05, ** P < 0.01.

    Article Snippet: Cytokine levels (IL-18(CSB-E07450h, Cusabio Wuhan, China), IL-1β (CSB-E08053h, Cusabio), IL-6(CSB-E04638h, Cusabio), IL-12(CSB-E04599h, Cusabio), IL-23(CSB-E08461h, Cusabio), and TNF-α (CSB-E04740h, Cusabio)) were determined using ELISA kits according to the manufacturer's protocol.

    Techniques: Enzyme-linked Immunosorbent Assay, Fluorescence, Flow Cytometry, TUNEL Assay, Staining, Western Blot, Expressing, Activity Assay

    AS-IV inhibits pyroptosis in podocytes treated with high glucose. (a) Effect of YVAD (Z-YVAD-FMK) on cell survival in podocytes exposed to high glucose. (b) The effect of AS-IV on expression of IL-1 β and IL-18 in podocyte supernatant. (c) TUNEL staining to detect DNA fragmentation in podocytes in vitro (200x magnification, n = 3). Scale bar: 100 μ m. (d) PI (propidium iodide)/Hoechst 33342 staining (200x magnification, n = 3). Scale bar: 50 μ m. (e) The effect of AS-IV on LDH (lactate dehydrogenase) release in podocyte supernatants. (f) Caspase-1/PI staining as assayed by flow cytometry. (g) GSDMD-N and caspase-1 immunofluorescence (630x magnification, n = 3). Scale bar: 75 μ m. (h) The effect of AS-IV on pyroptosis-related parameters in podocytes exposed to high glucose. Values are presented as mean ± SD ( n = 3, ∗ P < 0.05 and ∗∗ P < 0.01).

    Journal: Journal of Diabetes Research

    Article Title: Astragaloside IV Attenuates High-Glucose-Induced Impairment in Diabetic Nephropathy by Increasing Klotho Expression via the NF- κ B/NLRP3 Axis

    doi: 10.1155/2023/7423661

    Figure Lengend Snippet: AS-IV inhibits pyroptosis in podocytes treated with high glucose. (a) Effect of YVAD (Z-YVAD-FMK) on cell survival in podocytes exposed to high glucose. (b) The effect of AS-IV on expression of IL-1 β and IL-18 in podocyte supernatant. (c) TUNEL staining to detect DNA fragmentation in podocytes in vitro (200x magnification, n = 3). Scale bar: 100 μ m. (d) PI (propidium iodide)/Hoechst 33342 staining (200x magnification, n = 3). Scale bar: 50 μ m. (e) The effect of AS-IV on LDH (lactate dehydrogenase) release in podocyte supernatants. (f) Caspase-1/PI staining as assayed by flow cytometry. (g) GSDMD-N and caspase-1 immunofluorescence (630x magnification, n = 3). Scale bar: 75 μ m. (h) The effect of AS-IV on pyroptosis-related parameters in podocytes exposed to high glucose. Values are presented as mean ± SD ( n = 3, ∗ P < 0.05 and ∗∗ P < 0.01).

    Article Snippet: IL-1 β (KE10003; Proteintech) and IL-18 levels (ab216165; Abcam) in the podocyte supernatant were quantified using their respective commercial ELISA kits detected via a microplate reader.

    Techniques: Expressing, TUNEL Assay, Staining, In Vitro, Flow Cytometry, Immunofluorescence

    NLRP3 (NLR family pyrin domain containing 3) inhibitor blocks pyroptosis in high-glucose-treated podocytes. (a) The effect of MCC950 on TUNEL staining (200x magnification, n = 3). Scale bar: 100 μ m. (b) The effect of MCC950 on LDH release in podocyte supernatant. (c) The effect of MCC950 on the expression of IL-1 β and IL-18 in podocyte supernatants. (d) The effect of MCC950 on PI/Hoechst 33342 staining (200x magnification, n = 3). Scale bar: 50 μ m. (e) The effect of MCC950 on caspase-1/PI staining as assayed using flow cytometry. (f) The effect of MCC950 on pyroptosis-related proteins in podocytes exposed to high glucose. Values are presented as mean ± SD ( n = 3, ∗ P < 0.05 and ∗∗ P < 0.01).

    Journal: Journal of Diabetes Research

    Article Title: Astragaloside IV Attenuates High-Glucose-Induced Impairment in Diabetic Nephropathy by Increasing Klotho Expression via the NF- κ B/NLRP3 Axis

    doi: 10.1155/2023/7423661

    Figure Lengend Snippet: NLRP3 (NLR family pyrin domain containing 3) inhibitor blocks pyroptosis in high-glucose-treated podocytes. (a) The effect of MCC950 on TUNEL staining (200x magnification, n = 3). Scale bar: 100 μ m. (b) The effect of MCC950 on LDH release in podocyte supernatant. (c) The effect of MCC950 on the expression of IL-1 β and IL-18 in podocyte supernatants. (d) The effect of MCC950 on PI/Hoechst 33342 staining (200x magnification, n = 3). Scale bar: 50 μ m. (e) The effect of MCC950 on caspase-1/PI staining as assayed using flow cytometry. (f) The effect of MCC950 on pyroptosis-related proteins in podocytes exposed to high glucose. Values are presented as mean ± SD ( n = 3, ∗ P < 0.05 and ∗∗ P < 0.01).

    Article Snippet: IL-1 β (KE10003; Proteintech) and IL-18 levels (ab216165; Abcam) in the podocyte supernatant were quantified using their respective commercial ELISA kits detected via a microplate reader.

    Techniques: TUNEL Assay, Staining, Expressing, Flow Cytometry